Journal: Journal of Medicinal Chemistry
Article Title: Discovery of Sulfonamide Pantothenate Kinase Activators and Elucidation of the Role of Isoform Selectivity in Cellular Pantothenate Kinase Activation
doi: 10.1021/acs.jmedchem.5c03452
Figure Lengend Snippet: Correlation Analysis and Surface Plasmon Resonance. A. Correlation analysis of the difference between K i of PANK1β and PANK3 vs C3A CoA elevation. The green-to-red scale shows the difference in K i between PANK1β and PANK3, while the size of the circles indicates the level of CoA elevation. B–E Surface Plasmon Resonance trace showing the binding of 20 and 21 to PANK3 and PANK1β in the presence of 1 mM ATP. B. 20 with PANK3. C. 20 with PANK1β. D. 21 with PANK3. E. 21 with PANK1β. The green data line was fit to a kinetic model shown in the black line. The association ( K a ), dissociation ( K d ), binding equilibrium ( K D ) constants, residence time (RT), and response units (RU) are shown in each panel.
Article Snippet: Human C3A (HepG2/C3A) cells (ATCC CRL-10741) were used for cellular assays.
Techniques: SPR Assay, Binding Assay